Effect of strawberry dietary supplement over IL-10 and IL-12 in TNBS model of rat ulcerative colitis

In the last few years the study of the IBDs has shown an interaction between the immune response and the intestinal flora. In the ulcerative colitis we note a dysregulation in the immune response, and this is one of the causes of the evolution of the inflammatory process [1]. A group of molecules has been reported in the participation of this inflammatory process, such as the pro-inflammatory interleukin 12 (IL-12) and anti-inflammatory interleukin 10 (IL-10). Strawberry already has been reported as a source of some molecules groups with anti-inflammatory and antioxidant activity, such as hydrolizable tannins and flavan-3,4-diol, and others [2]. The aim of this study was to evaluate the effect of strawberry on the diet of TNBS model of rat colitis. Three groups of rats were used (n=8); non-colitic (NC) and control groups (C) did not receive treatment, and the treated groups were given a diet with lyophilized fruit 5g/kg of a diet with 17% of proteins. After two weeks, colitis was induced by intracolonic administration of TNBS (30mg), and fed for one more week. Biochemical parameters (IL-10 and IL-12) were evaluated. The administration of the strawberry diet has shown an intestinal immunoregulatory activity. This diet significantly increased production of IL-10 (136±19.8 versus 93. 8±4 pg/g tissue; p<0.01) when compared with TNBS group. But there's no alteration on IL-12 levels.

Acknowledgements: CNPQ and Capes.

References: [1] de la Lastra, C.A., Villegas, I. (2007) Biochem. Soc. Trans. 35:1156–1160.

[2] Seeram, N.P. et. al. (2006) Food Chem. 97:1–11.