Protection-, Salt-, and Metal-Free Syntheses of [n]-Shogaols by Use of Dimethylammonium Dimethyl Carbamate (DIMCARB) without Protecting Groups

Abstract

Shogaols, the pungent principle of ginger, exhibit interesting bioactivities. Practical preparation of shogaols is highly desired. Here we report the protection/deprotection-, salt-, and metal-free synthesis of shogaol in three steps by use of dimethylammonium dimethyl carbamate (DIMCARB), in which DIMCARB smoothly promoted Mannich-type condensation of the ketone donor with the aldehyde acceptor through the iminium cation intermediate.

shogaols - protection-free synthesis - salt-free synthesis - metal-free synthesis - cross Mannich-type condensation

References

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7

Typical Procedure Handling of this class of reaction is very simple. DIMCARB (7, 101 µL, 0.5 mmol) was added to the solution of vanillin (5a, 76.1 mg, 0.5 mmol) in CH₂Cl₂ (0.5 mL) at 25 ˚C. Gas was evolved. Acetone (12, 36.7 µL, 0.5 mmol) was added in a single portion. Stirring was continued for 48 h. The solvent mixture was acidified with 10% HCl aq (2 mL) and extracted with CH₂Cl₂ (3 × 3 mL). The combined organic fraction was dried with anhyd Na₂SO₄, filtrated, and solvent removed in vacuo. Purification with column chromatography (silica gel, hexane-EtOAc) gave the enone 13a (77.8 mg, 81%) as a pale yellow solid. Without extraction, the crude reaction mixture could be directly purified by column chromatog-raphy in decreasing to 56% chemical yield.

8

We further investigated decreasing amount of DIMCARB, but chemical yields also decreased in the Mannich-type condensation of vanillin(5a) with acetone(12). Amount of DIMCARB = 1.0 equiv: 81%; 0.5 equiv: 72%; 0.1 equiv: 41%. These results suggested that DIMCARB catalyzed the Mannich-type condensation; however, due to low catalytic ability, a stoichiometric amount of DIMCARB was used for the present reactions.

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Reactions in conventional solvents such as DMSO, DMF, MeCN, 1,4-dioxane, Et₂O, THF, MeOH, 2-PrOH, toluene, and CHCl₃ resulted in low chemical yields (2a: 0-19%) along with self-Mannich product in 6-42% yields.

12 Northrup AB. MacMillan DWC. J. Am. Chem. Soc.  2002,  124:  6798 

13

Typical Procedure
To a solution of the aldehyde (3a, 58.9 mg,0.3 mmol) in DIMCARB (7, 300 µL, 1.47 mmol) was added dropwise hexanal (15a, 110 µL,0.9 mmol) by use of syringe pump over 10 h at 25 ˚C. Stirring was continued for 48 h. The solvent mixture was acidified with 10% HCl aq (2 mL) and extracted with CH₂Cl₂ (3 × 3 mL). The combined organic fraction was dried with anhyd Na₂SO₄, filtrated, and solvent removed in vacuo. Purification with column chromatog-raphy (silica gel, hexane-EtOAc) gave [6]-shogaol 2a (66.4 mg, 80%) as a pale yellow liquid; registry number 555-66-8; R _f  = 0.51 (hexane-EtOAc = 70:30). ^¹H NMR (300 MHz, CDCl₃): δ = 0.89 (t, J = 6.8 Hz, 3 H, CH₃), 1.15-1.57 (m, 6 H, 3 × CH₂), 2.10-2.28 (m, 2 H, CH=CHCH ₂), 2.75-2.96 (m, 4 H, CH₂CH₂Ar), 3.87 (s, 3 H, OCH₃), 6.09 (dt, J = 15.8, 1.1 Hz, 1 H, COCH=CH), 6.68 (dd, J = 7.9, 1.7 Hz, 1 H, H-6), 6.71 (d, J = 1.7 Hz, 1 H, H-2), 6.82 (dt, J = 15.8, 6.9 Hz, 1 H, COCH=CH), 6.83 (d, J = 7.9 Hz, 1 H, H-5). ^¹³C NMR (75 MHz, CDCl₃): δ = 200.01 (C), 147.95 (CH), 146.49 (C), 143.95 (C), 133.19 (C), 130.29 (CH), 120.77 (CH), 114.35 (CH), 111.16 (CH), 55.74 (OCH₃), 41.80 (CH₂), 32.26 (CH₂), 31.16 (CH₂), 29.72 (CH₂), 27.58 (CH₂), 22.22 (CH₂), 13.72 (CH₃). GC (TD-17, T_inj = 250 ˚C, T_det = 250 ˚C, He = 0.5 kg/cm^², H₂ = 0.5 kg/cm^², air = 0.5 kg/cm^², T_i = 250 ˚C): t _R = 9.410 min; HPLC [Mightysil, hexane-2-PrOH (95:5), flow rate 1.0 mL/min, λ = 254 nm]: t _R = 13.575 min.