Horm Metab Res 2009; 41(8): 594-599
DOI: 10.1055/s-0029-1216374
Original Basic

© Georg Thieme Verlag KG Stuttgart · New York

Heat Shock Protein 27 Modification is Increased in the Human Diabetic Failing Heart

T. Gawlowski 1 [*] , B. Stratmann 1 [*] , I. Stork 1 , B. Engelbrecht 1 , A. Brodehl 1 , K. Niehaus 3 , R. Körfer 2 , D. Tschoepe 1 , H. Milting 2
  • 1Heart and Diabetes Center NRW, Ruhr-University Bochum for the Meta-Card Research Group, Diabetes Center, Bad Oeynhausen, Germany
  • 2Heart and Diabetes Center NRW, Ruhr-University Bochum for the Meta-Card Research Group, Erich & Hanna Klessmann-Institute, Bad Oeynhausen, Germany
  • 3Bielefeld University, Proteom and Metabolom Research, Faculty of Biology, Bielefeld, Germany
Further Information

Publication History

received 15.12.2008

accepted 19.02.2009

Publication Date:
21 April 2009 (eFirst)

Abstract

Chronic conditions like diabetes mellitus (DM) leading to altered metabolism might cause cardiac dysfunction. Hyperglycemia plays an important role in the pathogenesis of diabetic complications including accumulation of methylglyoxal (MG), a highly reactive α-dicarbonyl metabolite of glucose degradation pathways and increased generation of advanced glycation endproducts (AGEs). The aim of this investigation was to study the extent of the MG-modification argpyrimidine in human diabetic heart and in rat cardiomyoblasts grown under hyperglycemic conditions. Left ventricular myocardial samples from explanted hearts of patients with cardiomyopathy with (n=8) or without DM (n=8) as well as nonfailing donor organs (n=6), and rat cardiac myoblasts H9c2 treated with glucose were screened for the MG-modification argpyrimidine. The small heat shock protein 27 (Hsp27) revealed to be the major argpyrimidine containing protein in cardiac tissue. Additionally, the modification of arginine leading to argpyrimidine and the phosphorylation of Hsp27 are increased in the myocardium of patients with DM. In H9c2 cells hyperglycemia leads to a decrease of the Hsp27-expression and an increase in argpyrimidine content and phosphorylation of Hsp27, which was accompanied by the induction of oxidative stress and apoptosis. This study shows an association between diabetes and increased argpyrimidine-modification of myocardial Hsp27, a protein which is involved in apoptosis, oxidative stress, and cytoskeleton stabilization.

References

1 These authors contributed equally to the work.

Correspondence

H. Milting, PhD 

Heart and Diabetes Center

NRW

Ruhr-University Bochum

Georgstraße 11

32545 Bad Oeynhausen

Germany

Phone: +49/5731/97 35 10

Fax: +49/5731/97 24 76

Email: hmilting@hdz-nrw.de