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Horm Metab Res 2009; 41(7): 542-547
DOI: 10.1055/s-0029-1216344
Animals, Clinical

© Georg Thieme Verlag KG Stuttgart · New York

Insulin Resistance Induced by Glucosamine in Fructose-fed Rats

C-S. Chien 1 , 2 , S-C. Cheng 1 , H-T. Wu 3 , C-W. Tsao 4 , J-T. Cheng 3 , 5
  • 1Department of Orthopaedics, Chi-Mei Foundation Hospital, Yang Kang City, Tainan County, Taiwan, R.O.C.
  • 2Institute of Biomedical Engineering, College of Medicine, National Cheng Kung University, Tainan City, Taiwan, R.O.C.
  • 3Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, Tainan City, Taiwan, R.O.C.
  • 4Department of Nursing, Chung Hwa University of Medical Technology, Tainan County, Taiwan, R.O.C.
  • 5Department of Medical Research, Chi-Mei Foundation Hospital, Yang Kang City, Tainan County, Taiwan, R.O.C.
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Publikationsverlauf

received 24.12.2008

accepted 12.02.2009

Publikationsdatum:
16. April 2009 (online)

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Abstract

Glucosamine has been widely used to treat osteoporosis in clinic and it shows an effect on hexosamine biosynthetic pathway in glucose-induced insulin resistance. However, glucosamine and chronic hyperglycemia is not correlative. Thus, we used C2C12 cell line to carry out the uptake assay of 2-[14C]-deoxy-d-glucose and [3H]-glucosamine. Glucosamine inhibited the in vitro glucose uptake in a concentration-dependent manner. The glucose transporter GLUT4 prepared for [3H]-glucosamine uptake showed a concentration-dependent competition between glucose and glucosamine uptake. The effects of glucosamine on glucose tolerance and homeostasis model assessment (HOMA) were also determined in normal Wistar and fructose-fed rats. Both plasma glucose levels and/or HOMA index were not changed in normal rats treated with glucosamine as compared with the saline-treated control. However, we found that glucosamine exhibited an effect on the expression of farnesoid X receptor in liver to exacerbate the values of HOMA and accelerate the development of insulin resistance in fructose-fed rats. Thus, glucosamine should be applied with caution in type-2 diabetic patients.

Key words

FXR - glucosamine - glucose transporter GLUT4 - insulin resistance - glucose tolerance test

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Correspondence

Prof. J-T. Cheng

Institute of Basic Medical Sciences

College of Medicine

National Cheng Kung University

Tainan City

Taiwan 70101

R.O.C.

Telefon: +886/6/331 85 16

Fax: +886/6/238 65 48

eMail: jtcheng@mail.ncku.edu.tw

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