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Exp Clin Endocrinol Diabetes 1995; 103: 133-135
DOI: 10.1055/s-0029-1211410
Islet Cell Transplantation and Bioartificial Pancreas

© J. A. Barth Verlag in Georg Thieme Verlag KG Stuttgart · New York

Different Toxic Effects of Hydrogen Peroxide, Nitric Oxide, and Superoxide on Human, Pig, and Rat Islets of Langerhans

T. Wacker, H. Jahr, S. Weinand, H. Brandhorst, D. Brandhorst, D. Lau, B. J. Hering, K. Federlin, R. G. Bretzel
  • Third Medical Department, Justus-Liebig-University, Giessen, Germany
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Publication Date:
15 July 2009 (online)

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Summary

Susceptibility of islet cells to damage by hydrogen peroxide, superoxide, and nitric oxide was determined on islets isolated from humans, pigs, and rats. Islets were incubated for 20 hr at 37°C with different concentrations of hydrogen peroxide, hypoxanthine/xanthine oxidase, or nitroprusside sodium, respectively. Islet cell damage was then measured as trypan blue-uptake. Rat islets showed a higher sensitivity than human or pig islets to damage by reactive oxygen species or nitric oxide. These results indicate that pig islets may be a more suitable model than rat islets to study inflammatory islet cell damage in diabetes and clinical islet transplantation.

Key words

hydrogen peroxide - superoxide - nitric oxide - islet of Langerhans

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