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Exp Clin Endocrinol Diabetes 1990; 96(5): 234-237
DOI: 10.1055/s-0029-1211014
Short Communication

© J. A. Barth Verlag in Georg Thieme Verlag KG Stuttgart · New York

Different Response of Porcine Large and Small Luteal Cells to PRL in Terms of Progesterone and Estradiol Secretion in Vitro

E. L. Gregoraszczuk
  • Laboratory of Animal Endocrinology and Tissue Culture, Department of Animal Physiology, Institute of Zoology, Jagiellonian University, Kracow/Poland
Further Information

Publication History

1990

Publication Date:
16 July 2009 (online)

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Summary

The extent of progesterone and estradiol secretion by isolated either large or small luteal cells was examined during incubation of separate cell types in medium without and with 100 ng/ml PRL. Small and large luteal cells were isolated from early corpora lutea of pig by means of Ficoll continous gradient. The amount of progesterone released by large cells into the incubation medium was 5 fold higher than that by the small ones. In the presence of PRL large cells secreted more progesterone (1.5 fold) than the control ones. We did not observe any stimulatory effect of prolactin on progesterone secretion by small luteal cells. On the other hand, prolactin added to the medium decreased estradiol production by both cell types.

Key words

Large and small luteal cells - PRL - Progesterone secretion

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