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Exp Clin Endocrinol Diabetes 1989; 93(1): 45-51
DOI: 10.1055/s-0029-1210835
Original

© J. A. Barth Verlag in Georg Thieme Verlag KG Stuttgart · New York

Specific Radioimmunoassay of Alpha-Melanocyte-Stimulating Hormone in Rat Plasma

M. Vecsernyés, J. Julesz
  • Endocrine Unit of First Department of Medicine, Albert Szent-Györgyi Medical University (Head: Prof. F. Szarvas), Szeged, Hungary
Further Information

Publication History

1988

Publication Date:
16 July 2009 (online)

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Summary

A sensitive, specific and reproducible radioimmunoassay was developed for the measurement of alpha-melanocyte-stimulating hormone (α-MSH) in the blood plasma of rat. The assay method is based on a sensitive antiserum raised against α-MSH in rabbit. The serum is highly specific to α-MSH; a HPLC study of an extract of rat plasma showed that the immunoreactivity was given by α-MSH. The basal level of α-MSH, measured after a simple extraction with ethanol, was found to be 168.3 ± 16.3 pg/ml (x; ± SEM). Ether and lysine-vasopressin appeared to be potent stimuli for the peripheral release of α-MSH.

Key words

Radioimmunoassay - α-MSH - Ether stress - Lysine vasopressin

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