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Exp Clin Endocrinol Diabetes 1988; 91(2): 155-160
DOI: 10.1055/s-0029-1210738
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© J. A. Barth Verlag in Georg Thieme Verlag KG Stuttgart · New York

Diminished Adhesion of Endothelial Aortic Cells on Fibronectin and Collagen Layers after Nonenzymatic Glycation

S. Krantz, Maria Lober, M. Thiele, E. Teuscher
  • Institute of Biochemistry (Director: Prof. Dr. sc. nat. H. Zühlke) and Department of Pharmacy (Director: Prof. Dr. sc. nat. H. Wollmann) of the Ernst Moritz Arndt University Greifswald/GDR
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Publication History

1987

Publication Date:
16 July 2009 (online)

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Summary

Adhesion of bovine endothelial cells on fibronectin and collagen before and after nonenzymatic glycation in vitro has been studied. Nonenzymatic glycation of these proteins reduced their ability to bind endothelial cells. Furthermore, nonenzymatically glycated fibronectin failed to bind to normal and nonenzymatically glycated gelatin and to fibrin. So gelatin and fibrin Sepharoses can be used to separate highly glycated fibronectins from fibronectins with a low degree of nonenzymatic glucose substitution. Sodium dodecylsulfate polyacrylamide gel electrophoresis did not demonstrate a covalent cross-link between nonenzymatically glycated fibronectins.

These results present further evidences for the role of nonenzymatic glycation of proteins in the development of vascular complications in long-term diabetes and of atherosclerosis.

Key words

Nonenzymatic glycation - Endothelial cells - Fibronectin - Collagen - Gelatin

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