Horm Metab Res 2009; 41(7): 516-522
DOI: 10.1055/s-0029-1202813
Original Basic

© Georg Thieme Verlag KG Stuttgart · New York

Cinnamon Extract Attenuates TNF-α-induced Intestinal Lipoprotein ApoB48 Overproduction by Regulating Inflammatory, Insulin, and Lipoprotein Pathways in Enterocytes

B. Qin 1 , 2 , H. Dawson 1 , M. M. Polansky 1 , R. A. Anderson 1
  • 1U.S. Department of Agriculture, Agricultural Research Service, Beltsville Human Nutrition Research Center, Diet, Genomics, and Immunology Laboratory, Beltsville, Maryland, USA
  • 2Integrity Nutraceuticals International, Spring Hill, TN 37174, USA
Further Information

Publication History

received 08.12.2008

accepted 27.01.2009

Publication Date:
06 April 2009 (eFirst)

Abstract

We have previously reported that the obesity-associated proinflammatory cytokine, TNF-α, stimulates the overproduction of intestinal apolipoprotein (apo) B48 containing lipoproteins. In the current study, we have evaluated whether a water-soluble cinnamon extract [CE (Cinnulin PF®)] attenuates the dyslipidemia induced by TNF-α in Triton WR-1339 treated hamsters, and whether CE inhibits the oversecrection of apoB48-induced by TNF-α in enterocytes in a 35S labeling study. In vivo, oral treatment of Cinnulin PF® (50 mg per kg BW), inhibited the postprandial overproduction of apoB48-containing lipoproteins and serum triglyceride levels. In ex vivo 35S labeling studies, CE (10 and 20 μg/ml) inhibited the oversecretion of apoB48 induced by TNF-α treated enterocytes into the media. To determine the molecular mechanisms, TNF-α treated primary enterocytes isolated from chow-fed hamsters, were incubated with CE (10 μg/ml), and the expression of the inflammatory factor genes, IL1-β, IL-6, and TNF-α, insulin signaling pathway genes, insulin receptor (IR), IRS1, IRS2, phosphatidylinositol 3-kinase (PI3-K), Akt1 and phosphatase and tensin homology (PTEN), as well as the key regulators of lipid metabolism, cluster of differentiation (CD)36, microsomal triglyceride transfer protein (MTTP), and sterol regulatory element binding protein (SREBP)-1c were evaluated. Quantitative real-time PCR assays showed that CE treatment decreased the mRNA expression of IL-1β, IL-6 and TNF-α, improved the mRNA expression of IR, IRS1, IRS2, PI3K and Akt1, inhibited CD36, MTTP, and PTEN, and enhanced the impaired SREBP-1c expression in TNF-α treated enterocytes. These data suggest that a water extract of cinnamon reverses TNF-α-induced overproduction of intestinal apoB48 by regulating gene expression involving inflammatory, insulin, and lipoprotein signaling pathways. In conclusion, Cinulin PF® improves inflammation related intestinal dyslipidemia.

References

Correspondence

B. Qin, MD, PhD 

USDA-ARS-BHNRC-DGIL

Building 307C, Rm 215

10300 Baltimore Ave

Beltsville

20705 MD

USA

Phone: +1/301/504 52 53 (ext. 272)

Fax: +1/301/504 90 62

Email: Bolin.Qin@ars.usda.gov

Email: bolin@integritynut.com

R. A. Anderson, Ph.D. 

USDA-ARS-BHNRC-DGIL

Building 307C, Rm 222

10300 Baltimore Ave

Beltsville

20705 MD

USA

Phone: +1/301/504 80 91

Fax: +1/301/504 90 62

Email: Richard.Anderson@ars.usda.gov