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DOI: 10.1055/s-0029-1185478
© Georg Thieme Verlag KG Stuttgart · New York
Improved Chromatographic Assessment of Chinese Medicinal Products by Multi-Chemical Classes Analysis
Publication History
received Nov. 3, 2008
revised February 3, 2009
accepted February 5, 2009
Publication Date:
16 March 2009 (online)
Abstract
An improved strategy for the quality evaluation of proprietary Chinese medicines (PCM) in complex matrices is presented. It involves the simultaneous separating and displaying of multi-chemical classes and analytes within a single chromatographic elution. The developed fingerprints have the properties of better coverage of multi-chemical classes among the composing herbs. This comprehensive and more facile approach was illustrated for Lemai Keli, a commonly used PCM listed in the Chinese Pharmacopoeia with highly complex analytical matrices. Ten marker compounds belonging to monoterpene glycosides, chalcone glycosides, phenolic acids and phenolic carboxylic aldehyde groups of the analytes together with three common chromatographic peaks were successively separated and displayed within the chromatographic window as a unique HPLC‐PAD fingerprint. Rigorous method validation data demonstrated the suitability of the current method for quality assessment purposes in a highly complex PCM matrix. Additional chemometric treatment further demonstrated the uniqueness of the developed fingerprint, with a distinctive chemical profile compared to a highly similar PCM formulation (Kangen Karyu) from which it was considered to be impossible to differentiate when using the conventional single marker approach.
Key words
proprietary Chinese medicine - fingerprinting - Lemai keli - high performance liquid chromatography
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Dr. Kelvin Sze-Yin Leung
Department of Chemistry
Hong Kong Baptist University
Kowloon Tong
Kowloon
Hong Kong Special Administrative Region
People's Republic of China
Phone: + 85 2 34 11 52 97
Fax: + 85 2 34 11 73 48
Email: s9362284@hkbu.edu.hk