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Planta Med 2009; 75(8): 814-822
DOI: 10.1055/s-0029-1185440
Pharmacology
Original Paper
© Georg Thieme Verlag KG Stuttgart · New York

Effect of Seven Tricyclic Diterpenoids from Needles of Taxus media var. Hicksii on Stimulus-Induced Superoxide Generation, Tyrosyl or Serine/Threonine Phosphorylation and Translocation of Cytosolic Compounds to the Cell Membrane in Human Neutrophils

Jia Tong1 , 2 , Jincai Lu2 , Na Zhang2 , Haidong Chi1 , Koichi Yamashita1 , Masanobu Manabe1 , Hiroyuki Kodama1
  • 1Department of Anesthesiology and Critical Care Medicine, Kochi Medical School, Nankoku Shi, Kochi, Japan
  • 2Department of Phytochemistry, Shenyang Pharmaceutical University, Shenyang, P. R. China
Further Information

Publication History

received Nov. 6, 2008 revised January 19, 2009

accepted January 23, 2009

Publication Date:
13 March 2009 (online)

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Abstract

Taxol has been widely used as an anticancer drug for ovarian, breast, lung and prostate cancer. Some kinds of Taxus plants are widely distributed in the Northeast Asia region. We have isolated seven tricyclic diterpenoids, taxinine, taxagifine, 5-O-cinnamoyltaxacin I triacetate, 5-decinnamoyltaxinine J, 5-cinnamoyl-9-acetyltaxicin I, taxacin and taxol from the needles of Taxus media var. Hicksii, and investigated their effects on stimulus-induced superoxide generation and translocation of cytosolic compounds to the cell membrane in human neutrophils. Six tricyclic diterpenoids used in this experiment suppressed the superoxide generation induced by N-formyl-methionyl-leucyl-phenylalanine (fMLP) and arachidonic acid (AA) in a concentration-dependent manner. Taxinine significantly suppressed the superoxide generation induced by phorbol 12-myristate 13-acetate (PMA). The compounds also suppressed fMLP- and AA-induced tyrosyl or PMA-induced serine/threonine phosphorylation, and translocation of cytosolic compounds, p47phox, p67phox and Rac to the cell membrane in parallel with the suppression of the stimulus-induced superoxide generation.

Key words

Taxus media var. Hicksii - Taxaceae - superoxide - human neutrophils - phosphorylation - cytosolic compounds

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Prof. Hiroyuki Kodama

Department of Anesthesiology and Critical Care Medicine
Kochi Medical School

Nankoku-shi

Kochi 783–8505

Japan

Phone: + 81 8 88 65 59 37

Fax: + 81 8 88 65 59 37

Email: jm-hkodama@kochi-u.ac.jp

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