Planta Med 2009; 75(8): 785-791
DOI: 10.1055/s-0029-1185398
Original Paper
© Georg Thieme Verlag KG Stuttgart · New York

Evaluation of Immunomodulatory Activity of an Extract of Andrographolides from Andographis paniculata

Suresh R. Naik1 , Amolkumar Hule1
  • 1Prin. K. M. Kundnani College of Pharmacy, Rambhau Salgaonkar Marg, Cuff, Parade, Colaba, Mumbai, India (Affiliated with the University of Mumbai, India)
Further Information

Publication History

received Nov. 11, 2008 revised January 12, 2009

accepted January 18, 2009

Publication Date:
04 March 2009 (online)


The immunomodulatory activity of HN-02, an extract containing a mixture of andrographolides (i.e., andrographolide [88 ± 5 %] plus 14-deoxyandrographolide and 14-deoxy-11,12-didehydroandrographolide together [12 ± 3 %]) in a pure powder form was evaluated at 1.0, 1.5, and 2.5 mg/kg on different in vivo and in vitro experimental models. In a delayed-type hypersensitivity (DTH) mouse model, potentiation of the DTH reaction was observed after treatment with cyclophosphamide (CYP) and HN-02 individually. However, CYP potentiation of the DTH reaction was reversed by HN-02 pretreatment. Furthermore, HN-02 treatment elevated the depressed hemagglutination antibody (HA) titer and increased the number of plaque-forming cells (PFCs) in the spleen cells of mice that had been treated with CYP and challenged with sheep red blood cells (SRBC). Further, it was also found that HN-02 treatment stimulated phagocytosis in mice. A significant increase in total WBC count and relative weight of spleen and thymus was observed in mice during 30 days of treatment with HN-02. The present experimental findings demonstrate that HN-02 has the ability to enhance immune function, possibly through modulation of immune responses altered during antigen interaction, and to reverse the immunosuppression induced by CYP.


Suresh R. Naik

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