Planta Med 2009; 75(5): 512-516
DOI: 10.1055/s-0029-1185305
Natural Product Chemistry
Original Paper
© Georg Thieme Verlag KG Stuttgart · New York

New Constituents with iNOS Inhibitory Activity from Mycelium of Antrodia camphorata

Sien-Sing Yang1 , Guei-Jane Wang2 , Shiang-Yi Wang3 , Yu-Ying Lin4 , Yueh-Hsiung Kuo5 , Tzong-Huei Lee4
  • 1Department of Internal Medicine, Cathy General Hospital, Taipei, Taiwan
  • 2National Research Institute of Chinese Medicine, Taipei, Taiwan
  • 3School of Pharmacy, Taipei Medical University, Taipei, Taiwan
  • 4Graduate Institute of Pharmacognosy, Taipei Medical University, Taipei, Taiwan
  • 5Tsuzuki Institute for Traditional Medicine, College of Pharmacy, China Medical University, Taichung, Taiwan
Further Information

Publication History

received Sept. 8, 2008 revised Nov. 24, 2008

accepted Nov. 30, 2008

Publication Date:
02 February 2009 (online)

Abstract

In continuing our investigation on the bioactive constituents of mycelium of Antrodia camphorata, antroquinonol B (1), 4-acetyl-antroquinonol B (2), 2,3-(methylenedioxy)-6-methylbenzene-1,4-diol (3) and 2,4-dimethoxy-6-methylbenzene-1,3-diol (4) along with antrodin D (5) were isolated by the guidance of an inducible nitric oxide synthase (iNOS) inhibitory assay and identified on the basis of their spectroscopic analysis. The effect of these compounds on the inhibition of NO production in lipopolysaccharide (LPS)-activated murine macrophages was further evaluated. Compounds 4 and 5 significantly inhibited NO production without any cytotoxicity, the IC50 values being 32.2 ± 0.1 and 26.3 ± 1.6 µg/mL, respectively. Compounds 1 and 2 possessed greater effects on NO inhibition, with IC50 values of 16.2 ± 0.8 and 14.7 ± 2.8 µg/mL, respectively, but displayed cytotoxicity at considerably higher concentrations. Compound 3 showed the lowest percent cell viability of 45.5 ± 1.8 % as observed in treated cells at a concentration of 16.8 µg/mL.

Supporting information available online at http://www.thieme-connect.de/ejournals/toc/plantamedica

References

Dr. Tzong-Huei Lee

Graduate Institute of Pharmacognosy
Taipei Medical University

250 Wu-Xin Street

Taipei

Taiwan 110

ROC

Phone: + 88 62 27 36 16 61 ext. 61 56

Email: thlee@tmu.edu.tw

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