Int J Sports Med 2009; 30(6): 426-429
DOI: 10.1055/s-0028-1112144
Physiology & Biochemistry

© Georg Thieme Verlag KG Stuttgart · New York

Changes of ROS during a Two-day Ultra-marathon Race

N. Hattori 1 , T. Hayashi 2 , K. Nakachi 2 , H. Ichikawa 3 , C. Goto 4 , Y. Tokudome 5 , K. Kuriki 6 , H. Hoshino 7 , K. Shibata 8 , N. Yamada 9 , M. Tokudome 10 , S. Suzuki 3 , T. Nagaya 1 , M. Kobayashi 11 , S. Tokudome 3
  • 1Public Health, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan
  • 2Department of Radiobiology/Molecular Epidemiology, Radiation Effects Research Foundation, Hiroshima, Japan
  • 3Department of Health Promotion and Preventive Medicine, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan
  • 4Department of Health and Nutrition, Nagoya Bunri University, Inazawa, Japan
  • 5School of Nutritional Sciences, Nagoya University of Arts and Sciences, Nisshin, Japan
  • 6Department of Epidemiology and Prevention, Aichi Cancer Center Research Institute, Nagoya, Japan
  • 7Department of Health Promotion, Aichi Bunkyo Women's College, Inazawa, Japan
  • 8Department of Health Promotion, Kasugai Health Maintenance Center, Kasugai, Japan
  • 9Department of Nutrition, Tenshi College, Sapporo, Japan
  • 10Medical Center, Yokohama City University, Yokohama, Japan
  • 11Department of Musculoskeletal Medicine, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan
Further Information

Publication History

accepted after revision November 28, 2008

Publication Date:
06 February 2009 (online)


To assess oxidative stress (OS) induced by endurance exercise, concentrations of serum reactive oxygen species (ROS) were determined in 70 Japanese male amateur runners completing a two-day ultra-marathon race. Serum ROS levels were analyzed at three time points: before the race (baseline), after the 1st day race (mid-race), and after the 2nd day race (goal) (post-race). The means (SE) of ROS were 151.4(3.7) (U. CARR.), 168.7(4.4), and 156.8(4.4), respectively. Significant positive trends were noted between age and serum ROS concentrations at the three race points (p<0.05 for all). After adjusting for age, BMI and average monthly running distance, the baseline serum ROS concentrations were positively associated with completion times of the first-day race, in particular (p<0.05), suggesting that the concentrations may predict physical performance. The ROS production increased at mid-race (p<0.05), but the levels returned to baseline levels at post-race, indicating that an antioxidant defense system may develop post-race to reduce OS.



S. Tokudome

Public Health

Nagoya City University

Graduate School of Medical Sciences


Mizuho-cho, Mizuho-ku

467-8601 Nagoya


Phone: +81/52/853 81 74

Fax: +81/52/842 38 30

Email: [email protected]