Abstract
The fixed oil from the pods of Heterophragma adenophyllum Seem, has been studied for its fattty acid composition by the combined application
of solid–liquid countercurrent distribution of urea inclusion complexes of fatty acids
after being segregated into solid and liquid fractions by lead–salt method as well
as of the mixed acids and mixed methyl esters, quantitative column chromatography
and , spectrophotometry. The acids were identified by uni– and two dimensional reversed
phase paper partition and thin–layer Chromatographie techniques. The fatty acids as
found by different techniques were: lauric acid, 1.23–1.79; myristic acid, 1.21–1.60;
palmitic acid, 2.91–3.24; stearic acid, 7.66–8.50; arachidic acid, 0.94–1.39; oleic
acid, 16.88–19.55; linoleic acid, 30.02–31.22 and linolenic acid 35.29–38.79 %. The
chief ingredients of unsaponofiable matter were found to be: (I) octacosane or an
equimolecular mixture of C27 and C2g paraffins, (II) unidentified C6H5–C18H37 hydrocarbon, (III) myricyl alcohol, (IV) friedelin, (v) β–amyrin, (VI) unidentified
triterpenoid, C30H50O2 (m. p. 230–31°) and (VII) β–sitosterol.
