VERLAUF VON PHENYLALANIN–AMMONIUM–LYASE–AKTIVITÄT, ANTHOCYAN– UND CHLOROGENSÄUREBILDUNG IN VERSCHIEDENEN ZELLSTÄMMEN VON DAUCUS CAROTA

Abstract

The enzyme phenylalanine-ammonialyase (PAL) could be detected in six cell lines of Daucus carota L. which differ in their ability to synthetize anthocyanins. In those cell lines which do not produce anthocyanins in the dark, PAL activity is enhanced by light. With one exception this is paralleled by anthocyanin biosynthesis. 2.4–dichlorophenoxy acetic acid delays PAL activity and anthocyanin or chlorogenic acid accumulation. In three other cell lines which are able to produce anthocyanins under special conditions in the dark, too, PAL activity is not enhanced by light, although anthocyanin production is increased or in one case even induced. It is concluded that at least in those latter cell lines anthocyanin biosynthesis is not regulated by PAL but by an enzyme which is located later on in the biosynthetic pathway.