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Planta Med 1976; 29(2): 160-164
DOI: 10.1055/s-0028-1097646
© Georg Thieme Verlag Stuttgart · New York

ZUR METHODE DES ZELLAUFSCHLUSSES FÜR LIPIDUNTERSUCHUNGEN BEI DEN HEFEN ENDOMYCOPSIS VERNALIS UND TORULOPSIS UTILIS

Views of methods of cell-disruption for investigation of the lipids of the yeasts Endomycopsis vernalis and Torulopsis utilisH. Fischer, H. Kating
  • Institut für Pharmazeutische Biologie der Philipps–Universität Marburg a. d. Lahn und Institut für Pharmazeutische Biologie der Friedrich–Wilhelms–Universität Bonn
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Publication Date:
13 January 2009 (online)

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Abstract

Three methods of cell-disruption followed by extraction with petrolether were tested on the yeasts En do my cop si s vernalis and Torulopsis utilis in order to investigate the quantities of their lipids and their qualitative composition. Most of the lipids were extracted from Endomycopsis vernalis after lyophilisation followed by grinding the my eel with diatomaceous earth, from Torulopsis utilis after disrupting the cells with a glas bead cell-homogenisator.

The acid degree of the lipidextracts of the two yeasts was highest after cell-disruption with ultrasound but lowest after lyophilisation followed by grinding the mycel with diatomaceous earth. A high acid degree indicates a high degree of disruption of lipid compounds caused by the different celldisruption methods.