High Performance Liquid Chromatographie Determination of Ginseng Saponin by Ultraviolet Derivatisation

H. Besso; Y. Saruwatari; K. Futamura; K. Kunihiro; T. Fuwa; O. Tanaka

doi:10.1055/s-0028-1097332

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Planta Med 1979; 37(11): 226-333
DOI: 10.1055/s-0028-1097332

Research Articles

High Performance Liquid Chromatographie Determination of Ginseng Saponin by Ultraviolet Derivatisation

H. Besso, Y. Saruwatari, K. Futamura, K. Kunihiro, T. Fuwa¹ , O. Tanaka²

¹Wakunaga Pharmaceutical Co., Ltd. and
²Institute of Pharmaceutical Science, Hiroshima University School of Medicine, Japan.

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Publication History

Publication Date:
13 January 2009 (online)

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Abstract

Ginsenosides Rb₁, b₂, c, d, e and g₁ quantitatively reacted with benzoyl chloride in pyridine to afford the respective derivatives having strong UV absorption. The UV–derivatized saponins were separated by high performance liquid chromatography (HPLC), and the saponin–contents of the various Ginseng varieties were determined. It is possible to distinguish white Ginseng from the root hairs of Ginseng from the root hairs of Ginseng by the pattern analysis of HPLC.

Key Word Index

Panax ginseng - Araliaceae - UV–Derivatization of Saponin - High Performance Liquid Chromatography