The ability of anti-insulin serum to bind insulin in plasma is significantly altered
by the in vitro addition of arginine, leucine and epinephrine as well as by the final
concentration of albumin in the plasma-buffer mixture.
The amino acids (50 µg per ml) or albumin interfere with binding and this results
in artefactually high insulin values. Epinephrine (0.125 µg per ml) produces false
low insulin measurements.
Anti-Insulin Serum - Amino Acids - Epinephrine - Insulin Radioimmunoassay