Adsorbent Techniques for the Separation of Antibody-Bound from Free Peptide Hormones in Radioimmunoassay

G. M. A. Palmieri; R. S. Yalow; S. A. Berson

doi:10.1055/s-0028-1094150

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Horm Metab Res 1971; 3(5): 301-305
DOI: 10.1055/s-0028-1094150

Originals

Adsorbent Techniques for the Separation of Antibody-Bound from Free Peptide Hormones in Radioimmunoassay

G.M. A. Palmieri [*] , R. S. Yalow , S. A. Berson

Radioisotope Service, Veterans Administration Hospital, Bronx, N.Y.
Department of Medicine, Mt. Sinai School of Medicine of the City University of New York, New York, N.Y., USA

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Publikationsdatum:
07. Januar 2009 (online)

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Abstract

Several adsorbing agents (Talcum powder tablets, Quso, uncoated wood charcoal, Ficoll-coated charcoal) for separation of antibody-bound from free hormone in radioimmunoassays of insulin, growth hormone, ACTH, parathyroid hormone and calcitonin were compared. Under suitable conditions, the several adsorbing agents gave similar results with most of the hormones tested.

Key words

Radioimmunoassay - Peptide Hormones - Insulin - Growth Hormone - ACTH - Parathyroid Hormone - Calcitonin - Adsorbing Agents

1 Present address: Veterans Administration Hospital and Oklahoma Medical Research Foundation, Oklahoma City, Oklahoma, USA