Abstract
A study was made of the acute effects of growth hormone on the rate of leucine incorporation
into protein of various tissues of hypophysectomized rats in vivo. The rats were injected
with bovine growth hormone intravenously or intraperitoneally for various periods
of time, and then the rate of protein synthesis was assessed by pulse-labeling proteins
with L-leucine-14 C administered intravenously through a tail vein, Growth hormone stimulated leucine
incorporation into the total TCA-precipitable protein of thigh muscles 30 min, but
not 15 min, after its administration. The stimulatory effect was still evident at
6 and 24 hr after hormone injection. These observations are similar to those previously
made with diaphragm muscle. In contrast, no stimulation of leucine incorporation into
proteins of the heart was seen 0.25, 0.5, 1, 3, 6, or 24 hr after a single injection
of a large dose of growth hormone. In another experiment, the rate of leucine incorporation
into proteins of several tissues was assessed by the pulse-labeling technique after
30 min of exposure to growth hormone in vivo. Once again, the hormone increased (by
67%) the rate of leucine incorporation in the protein of both the thigh muscle and
the diaphragm. While no significant stimulation of protein labeling was evident in
the thymus, kidney or brain at this time, growth hormone enhanced leucine incorporation
into hepatic protein by 150%. Neither thymidine factor activity nor the concentration
of immunoreactive insulin was increased in the plasma of hypophysectomized rats 30
min after they received an injection of growth hormone. Thus, these results suggest
that the acute stimulatory effects of pituitary growth hormone on protein synthesis
in skeletal and diaphragmatic muscle and in the liver are not mediated by thymidine
factor or insulin.
Key words
Pituitary Growth Hormone - Protein Synthesis - Muscle - Liver - Thymidine Factor -
Plasma Insulin
1 Publication No. 2002, Division of Basic Health Sciences, Emory University.
2 Recipient of a postdoctoral fellowship (5 F02 HD-44387) from the NIH. Present address:
Department of Physiology and Biophysics, University of Tennessee Medical Units,
Memphis, Tennessee 38103, USA