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Horm Metab Res 1974; 6(4): 303-306
DOI: 10.1055/s-0028-1093834
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© Georg Thieme Verlag KG Stuttgart · New York

Lipolytic Effects of Sheep β-Lipotropin in Rat Adipose Tissue: Interaction with Theophylline and Dihydroergotamine[*]

A.  Kowadlo Silbergeld , Z.  Laron
  • Pediatric Metabolic and Endocrine Institute and Rogoff Wellcome Medical Research Institute, Beilinson Hospital, Department of Physiology and Pharmacology, Tel Aviv University Medical School, Israel
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Publication History

Publication Date:
08 January 2009 (online)

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Abstract

Lipolytic effects produced by permissive doses (0.025-1.0 µg/ml) of sheep β-lipotropin (βLPH), were studied in adipose tissue and isolated cells of the rat. Preincubation of tissue segments with βLPH for three hours augmented the glycerol release due to theophylline subsequently added in fresh medium. In isolated fat cells, incubation of βLPH, together with dihydroergotamine (DHE) and dexamethasone (DEX), stimulated the release of FFA to a level which exceeded that produced by any other combination of agents in the experiment. This effect was suppressed by cycloheximide. The effects described for βLPH are similar to those produced by growth hormone preparations. The data presented suggests that the stimulation of lipolysis by βLPH in the rat may be a function of protein synthesis.

Key words

Lipolysis - Rat Adipose Tissue and Cells - Sheep βLipotropin - Human Growth Hormone - Theophylline - Dihydroergotamine - Dexamethasone

1 This work is part of a M.Sc. thesis of A.K.S. to be submitted to the Tel Aviv Unversity Medical School.

1 This work is part of a M.Sc. thesis of A.K.S. to be submitted to the Tel Aviv Unversity Medical School.

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