Abstract
The effects of insulin and procaine-hydrochloride on the protein kinase activation
ratio and the phosphatase(s) activity have been studied in rat adipose tissue. The
relationship between the changes in the activities of these enzyme preparations and
tissue cyclic-AMP has also been investigated.
Insulin decreased the protein kinase activation ratio and increased the phosphatase(s)
activity prior to its effect on lipolysis. Such changes were not associated with comparable
alterations in tissue cyclic-AMP. The changes in protein kinase and phosphatase activities
correlated both in time and magnitude with the inhibition of lipolysis.
Procaine hydrochloride did not affect the tissue accumulation of cyclic-AMP in response
to adrenalin, yet it produced similar changes to insulin in affecting the protein
kinase activation ratio, phosphatase(s) activity and inhibition of lipolysis.
These results suggest a common mechanism for the anti-lipolytic effect of insulin
and procaine which involves an alteration in cellular calcium distribution and is
independent of their effects on tissue cyclic-AMP.
Key words
Cyclic-AMP - Adipose Tissue - Insulin - Procaine HCl - Glycerol Release - Adrenalin
- Lipolysis
1 Presented in part to the British Diabetic Association, Spring meeting 13-14 April
1973.
