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Horm Metab Res 1974; 6(6): 443-447
DOI: 10.1055/s-0028-1093820
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© Georg Thieme Verlag KG Stuttgart · New York

Untractable Hypoglycemia in an Infant: Studies on Pancreas Insulin and Glucagon

F.  Rancon , M.  Laburthe , G.  Rosselin , P.  Freychet
  • Unité de Recherche de Diabétologie et d'Etudes Radio-Immunologiques des Hormones Protéiques U.55, (Institut National de la Santé et de la Recherche Médicale), Hôpital Saint-Antoine, Paris, France
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Publikationsdatum:
07. Januar 2009 (online)

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Abstract

Insulin and glucagon were extracted from the pancreas of a 2-month old child with untractable hypoglycemia. High contents of insulin (680 µg/g of wet weight) and of glucagon (84 µg/g of wet weight) were found. After gel filtration, the different fractions were analysed by radioimmunosassy, (RIA) and radioreceptorassay (RRA) for insulin and glucagon. The proinsulin fraction represented 5.4% and 1.6% of the total insulin in the RIA and the RRA, respectively. Five percent of the glucagon was found as a larger size component. The insulin and glucagon fractions behaved identically to the reference hormones in both the RIA and the RRA; the glucagon fraction was as potent as the reference glucagon in stimulating adenylate cyclase in liver membranes.

Key words

Human Pancreas - Infant Hypoglycemia - Insulin - Glucagon - Adenylate Cyclase - Liver Membranes - Receptors - Radioimmunoassay