Abstract
Using different decay data of "in vivo" pulse-labeled protein, the half-life values
of different fractions from mouse liver, heart, striated leg muscle, jejunum and epidydimal
fat pads were calculated by considering labeled intracellular amino acid reutilization.
This was accomplished by substracting the pool amino acid decay rates from the protein
decay rates. The extent of reutilization of these labeled amino acids is considerable
as shown by the differences found between the corrected and uncorrected half-life
calculations. The half-life values obtained for total protein in liver, striated muscle,
heart, duodenum and epidydimal fat pad were: 0.57, 1.81, 2.13, 0.68 and 0.42 days,
respectively.
Key words
Protein Turnover - Amino Acid Pools - Protein Synthesis - Mice
