Download PDF
Horm Metab Res 1978; 10(4): 337-340
DOI: 10.1055/s-0028-1093426
Originals

© Georg Thieme Verlag KG Stuttgart · New York

Effect of Hormone on Human Mammary Duct in Vitro

L. J. van Bogaert
  • Experimental Pathology & Cytology Unit, University of Louvain, Belgium
Further Information

Publication History

Publication Date:
23 December 2008 (online)

Also available at
 PDF Download  Permissions and Reprints

Abstract

Human mammary ducts were maintained in median-term organ culture. DNA synthesis was assessed by the uptake of 3H-thymidine in epithelial cells; labeling indices were counted on autoradiographies. DNA synthesis grossly remained between starting values in the basic conditions without hormones. Insulin (5 µg/ml) did not stimulate the uptake of the isotope as compared with the controls. Estradiol (0.001 µg/ml) and progesterone (1 µg/ml) highly significantly enhanced DNA synthesis; subsequent waves did occur.

Key words

Organ Culture - Human Mammary Duct - Insulin - Estradiol - Progesterone