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Horm Metab Res 1979; 11(5): 366-370
DOI: 10.1055/s-0028-1092740
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© Georg Thieme Verlag KG Stuttgart · New York

Propranolol Inhibits the In Vitro Conversion of Thyroxine into Triiodothyronine by Isolated Rat Liver Parenchymal Cells

C.J.F.  van Noorden , W. M. Wiersinga , J. L. Touber
  • Division of Endocrinology, Department of Medicine, University Hospital "Wilhelmina Gasthuis", Amsterdam, The Netherlands
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Publication History

Publication Date:
17 December 2008 (online)

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Abstract

A model for the in vitro study of the conversion of thyroxine into triiodothyronine using isolated rat liver parenchymal cells is described.

Isolated liver cells (mean protein content 18 mg/ml) convert approximately 0.8% of 1.3 µM exogenously added T4 into T3 during thirty minutes incubation. Carbimazole (50 µM) has no effect on the conversion process, whereas propylthiouracil (50 µM) inhibits the conversion. The β-adrenoceptor blocking agent propranolol lowers the conversion ratio when added in concentrations of 580 and 1160 µM, but has no inhibitory effect when 290 µM is added.

Key words

Isolated Rat Liver Cells - Propranolol - Conversion of Thyroxine - Triiodothyronine

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