Planta Med 2008; 74 - PK12
DOI: 10.1055/s-0028-1084981

Genetic diversity of Peucedanum japonicum using Internal Transcribed Spacer (ITS) sequence

YH Kim 1, JA Ryuk 1, BS Ko 1, JW Lee 1, SE Oh 2, MY Lee 1
  • 1Korea Institute of Oriental Medicine, Daejeon, 305–811, Korea
  • 2Department of Biological Sciences, Konkuk University, Seoul 143–701, Korea

Peucedanum japonicum Thunberg (Umbelliferae) is a medicinal plant, found as native plant in the beaches of East Asian countries. In Korea, Peucedani Radix, named Sik-Bang-Poong (SBP), is used in many prescriptions for the treatment of analgesic symptoms [1, 2]. It is difficult to find a systematic study on vegetation for the distribution and Peucedanum japonicum growing environment of their habitats. According to the result of sequences of the internal transcribed spacer (ITS) region, the specimens were divided into two groups(SBP-1, SBP-2) even though they grow spontaneously in the same region in Korea. The SBP-1 group has showed 99% homology and 2 bp difference as compared to that of NCBI Genbank (DQ270201) which is registered. And the SBP-2 group was classified into another group that showed 4 bp difference to that of SBP-1 group. We have registered to the NCBI Genbank as EU224273(SBP-1) and EU224274(SBP-2), which are new sequences of P. japonicum. In this study, we have observed the genetic diversity of P. japonicum and the result could be used to study of the genetic variation and characterization.

Acknowledgements: This research was supported by a grant from the Korea Food and Drug Administration

References: 1. Okuyama, E. et al. (2001) Chem. Pharm. Bull. 49: 154–160.

2. Kang, S.K. et al. (2002)J. Vet. Sci. 3: 343–349.