Comparative studies regarding the influence of the esters and the ethanol amides of the fatty acids from extra-virgin Oleum Olivae on the experimental ulcers and on the gastric activity on rat
We measured the protective effect of the esters and ethanolamides of the fatty acids from extra-virgin Oleum Olivae on the experimental gastric ulcers provoked by indomethacin and on the gastric secretion on rat. In this study, male Wistar rats, weighting 200±10g were treated with indomethacin and combinations of indomethacin with ethanolamides, methylic esters of the fatty acids from the olive oil and indomethacin with extra-virgin Oleum Olivae. After four treatment days the pilory was ligatured and after two hours the animals were sacrificed under anaesthesia and the gastric secretion was collected.
The gastric mucosa was examined with lens marking the provoked ulcers, depending on their length. The gastric acidity of the collected gastric secretion was measured the by Töpfer Linosier method . The chronic administration of indomethacin produced frequent ulcers. The statistical interpretation of the results was made using Student „t“ test . The treatment with the fatty acids ethanolamides in the presence of indomethacin reduced the ulcers number by 90.0% compared with indomethacin and methylic esters, reduced by 53.6%. The gastric acidity of the animals treated with indomethacin and fatty acids ethanolamides decreased by 60.7% compared with the animals treated with indomethacin only, and by 38.1% for the methylic esters and indomethacin.
In conclusion, the present study shows that the ethanolamides of the fatty acids from the extra-virgin Olive olivae presented a protective effect on the ulcers provoked by indomethacin.
References:1 Mihele, D. (2007) Biochimie clinicã. Metode de laborator. ed. a III-a, Ed. Medicala, Bucureşti.
2 Joean, D. (1997) Farmacia 45: 55–63.