Planta Med 2008; 74 - PH4
DOI: 10.1055/s-0028-1084849

Inhibition of vascular endothelial growth factor by highbush blueberry fruit (Vaccinium corymbosum v bluecrop) in Eahy 926 and U937 cell lines

A Der Marderosian 1, R Kota 1, J Porter 1, D Morel 1, J Mckee 1, A Howell 2
  • 1University of the Sciences in Philadelphia, Philadelphia, PA, 19104, U.S.A
  • 2Blueberry & Cranberry Research Center, Chatsworth, NJ, U.S.A

Previous studies have shown that Blueberries possess anti-angiogenic, anti-infective, anti-inflammatory, anti-carcinogenic, and anti-Alzheimer's properties (Bagchi et al. 2004, Joseph et al. 2003). Whole Blueberry Extract (WBE) was prepared from Vaccinium corymbosum L Bluecrop, by macerating the whole crushed blueberries in 80% acetone/water. The above WBE fraction was lyophilized and tested using the Enzyme Linked Immuno-Sorbent Assay (ELISA) for Vascular Endothelial Growth Factor (VEGF) in U937 and Eahy926 cell lines. The WBE fraction of Vaccinium corymbosum L bluecrop was found to inhibit VEGF in both the cell lines and the inhibitory activity was proportional to its concentration. These observations indicate that Whole Blueberry Extract possesses potent inhibitory activity on VEGF that results in anti-angiogenic property which helps in the protective action against certain cancers. Cytotoxicity studies were also performed on these cell lines for the effective inhibitory concentrations of the WBE fraction and were found to be non toxic. The above WBE fraction was further fractionated into different polyphenolic fractions and the highly active fraction was determined.

References: 1. Bagchi, D.et al. (2004) Biochemistry (Mosc). 69:75–80.

2. Joseph, J.A et al.. (2003) Nutr Neurosci. 6:153–62.