Wound healing and antioxidant activities of extracts from Musa paradisiaca L. peel
The process of wound healing involves a variety of complex processes such as inflammation, cell proliferation and contraction of the collagen lattice formed. In addition, the healing process may be hampered by the presence of oxygen free radicals or microbial infection . The process of wound healing is promoted by several plants natural products, which are composed of active principles such as triterpenes, alkaloids and biomolecules. These agents usually influence one or more phases of the healing process .
MeOH and hexane extracts from M. paradisiaca peel were obtained and their biological activity evaluated (antioxidant and wound healing) on the regenerative process of the epithelial tissue. To validate the ethnotherapeutic claims of the plant in skin diseases, wound healing activity was studied, besides antioxidant activity to understand the mechanism of wound healing activity. The antioxidant potential of the plant and radical analysis was carried out by DPPH method. The 2 extracts showed a good inhibition of DPPH radical, the hexane has an activity of 94.25% and MeHO 87.33% at a concentration of 125µg/mL compared with BHT 43.22% as a control. Finally to assess the healing pattern the extracts were topically applied on the wound. Wound reduction rate and histological analysis were evaluated. Wound closure was significantly more advanced in the treated groups MeOH 94.62%, hexane 88.39% compared with control groups 81.75% at 15 days. The results suggest that extracts obtained with MeOH has potential to stimulate the healing process in a close relation to antioxidant properties more that hexane extracts. MOH extracts were analyzed by HPLC-MS and sitoindosides were found as in Musa paradisiaca pulp which is reported with anti-ulcer properties.
Acknowledgement: This work was partly supported by Laboratorios Cryopharma and COECYTJAL grant FOMIX-JAL 2004–01–13.
References: 1. Mensah, AY. et. al. (2001)J. Ethnopharmacol 77: 219–226; 2. Suguna et al. (2002) Phytother. Res. 16: 227–231