Structure elucidation of components with inhibitory activities of atopic dermatitis from Actinidia arguta
Atopic dermatitis is a common, chronic fluctuating skin disease. Over the last 30 years, prevalence of atopic dermatitis and other atopic allergic disorders has been continuously increased . Therefore, the importance of research regarding the treatment of atopic dermatitis is also increasing. The fruits of Actinidia arguta (AA) were generally used as a traditional medicine for skin diseases, diuretic effect, diabetes mellitus and osteoporosis. Recently, it has been reported that AA possesses both in vivo and in vitro anti-inflammatory effects . Although Actinidia arguta has high inhibitory effects on allergic diseases, the active compounds have not been identified. In this study, activity-guided isolation of the extract of the dried fruits from AA using in vitro ELISA assay for IL-4 and luciferase assay , led to the purification of five fatty acid compounds. The active compounds were identified as α-linolenic acid (C18:3), linoleic acid (C18:2), α-linolenic acid ethyl ester (ethyl linolenate, C18:3), linoleic acid ethyl ester (ethyl linoleate, C18:2) and stearic acid ethyl ester (ethyl stearate, C18:0), respectively. The results suggest that these five active compounds are potent inhibitors of PMA- and A23187-induced IL-4 promoter activity and IL-4 production in RBL-2H3 cells without cell toxicity.
Acknowledgements: Dong-A Pharmaceutical Company, Ltd.
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