Planta Med 2008; 74 - PC144
DOI: 10.1055/s-0028-1084662

Validated HPTLC method for the identification of Hoodia gordonii

V Widmer 1, E Reich 1, A DeBatt 1, A Ankli 1
  • 1CAMAG Laboratory, Sonnenmattstrasse 11, CH-4132 Muttenz, Switzerland

An HPTLC method for rapid and reliable identification of Hoodia gordonii raw material in a cGMP environment was developed. Based on authenticated botanical reference material the method allows discrimination of Hoodia gordonii from the closely related species Hoodia currorii and Hoodia parviflora as well as from the common adulterant prickly pear cactus (Opuntia ficus-indica). The mobile phase is chloroform, methanol, water 70:30:3 (v/v) and the stationary phase is HPTLC silica gel. The method was validated with respect to specificity, reproducibility and robustness.

Acknowledgements: We thank Roy Upton (American Herbal Pharmacopoeia AHP) and Bruno Erni-Rodman (Botanic Garden, Basel, Switzerland) for providing numerous plant samples. For donating samples of P57 we are grateful to Brian Schaneberg (ChromaDex) and Ikhlas Khan (University of Mississippi).