Planta Med 2008; 74 - PC100
DOI: 10.1055/s-0028-1084618

Simultaneous determination of atropine and scopolamine in Hyoscyamus arachnoideus by HPLC

N Hosseini 1, SN Ebrahimi 2, P Salehi 2, B Asghari 2, M Ahmadi 2, H Salhi Arjmand 1
  • 1Department of Medicinal Plant, Faculty of Agriculture, Arak University, Arak, Iran
  • 2Department of Phytochemistry, Medicinal Plants and drugs Research Institute, Shahid Beheshti University, Evin, Tehran, Iran

Tropane alkaloids are natural compounds having in common, the 8-aza-bicyclo [3.2.1] octane structure. They mainly occur in the Solanaceae, Erythroxylaceae, and Convolvulaceae families [1]. A reverse phase high-performance liquid chromatography equipped with UV-PDA detector, and C18 Lichrospher 100 RP8, 250×4.6mm colum was used for the analysis of the main tropane alkaloids, scopolamine and atropine, in the roots, aerial parts and seeds of Hyoscyamus arachnoideus. The samples were analyzed using a buffer containing 50 mM potassium dihydrogen orthophosphoric acid adjusted to pH 3.0 by orthophosphoric acid: Acetonitrile (80: 20 v/v). The mobile phase was pumped at a constant flow rate of 1.4 mL/min and detection was carried out at a wavelength of 215nm. Obtained results showed that scopolamine was the main alkaloid in the seeds while atropine was the major alkaloid in the roots. The linearity of the method was in the range of 4–400µg/mL for atropine and 0.8–80µg/mL for scopolamine. LOD and LOQ values were 5.15 and 17.4µg/mL for atropine and 1.92 and 6.4µg/mL for scopolamine.

1. Bieri S., et. al., (2006) Phytochem. Anal. 17: 78–86.