Planta Med 2008; 74 - PC80
DOI: 10.1055/s-0028-1084598

Simultaneous determination of lignans, aristolactam alkaloids and flavonoids in the Saururus chinensis by validated HPLC-DAD-MS/MS

KY Lee 1, JH Park 1, EJ Cho 2, SH Kim 2, YC Kim 1, SH Sung 1
  • 1College of Pharmacy and Research Institute of Pharmaceutical Science, Seoul National University, Seoul 151–742, Korea
  • 2Institute for Life Science, Elcom Science Co. Ltd., Seoul 151–742, Korea

Saururus chinensis (Saururaceae) has been used in Korean folk medicine for the treatment of edema, jaundice and gonorrhoea. A method coupling HPLC-DAD and ESI-MS/MS was established for the characterization of lignans, aristolactam alkaloids and flavonoids in the S. chinensis. Ten compounds were unambiguously identified comparing experimental data for retention time, UV spectra and MS/MS fragmentation patterns with those of the authentic compounds: rutin (1), quercetin 3-O-β-D-glucopyranosyl(1→2)-O-α-L-rhamnopyranoside (2), quercetin 3-O-β-D-glucuronide (3), quercitrin (4), quercetin (5), aristolactam AII (6), saurolactam (7), sauchinone (8), saucereneol A (9) and manassantin A (10). Furthermore, a HPLC-DAD method has been performed for the simultaneous determination of five marker constituents, rutin (1), quercetin 3-O-β-D-glucopyranosyl(1→2)-O-α-L-rhamnopyranoside (2), quercetin 3-O-β-D-glucuronide (3), quercitrin (4), and sauchinone (8) in extract of aerial part of S. chinensis collected in different seasons of harvest.

Acknowledgements: PF06200–00 from Plant Diversity Research Center of the 21st Century Frontier Research Program funded by the Ministry of Science and Technology, Korea.

References: 1. Ahn, M.J. et al. (2008)J Pharm Biomed Anal, 46:258–66.

2. Sung, S.H. et al. (2000) Biol Pharm Bull, 23:666–8.