Planta Med 2008; 74 - PC79
DOI: 10.1055/s-0028-1084597

Optimization of the extraction procedures and HPLC characterization of Helichrysum plicatum DC flower extracts

T Jankovic 1, D Bigovic 1, G Zdunic 1, K Savikin 1, N Menkovic 1
  • 1Institute for Medicinal Plants Research, T. Košćuška 1, 11000 Belgrade, Serbia

The genus Helichrysum (Asteraceae) is represented by approximately 300 species in the world, widely distributed in Europe. Some members of this genus have cholagogue and choleretic activity and stimulate the secretion of gastric juice [1]. Helichrysum plicatum has been used in Serbian and Macedonian folk medicine for treatment of gastric and hepatic disorders. Moreover, antidiabetic, antioxidant and antibacterial activity were documented [2, 3]. The medicinal properties of this genus are mainly attributed to the presence of flavonoids [2]. In our study 13 different extracts were prepared from air – dried flowers of H. plicatum. Type of extragens (ethanol/water in different ratio), degree of comminution and reextraction solvent (ethanol/ethylacetate in different ratio) were varied. HPLC methods were developed for non-hydrolized (NHE) and hydrolized extracts (HE). In NHE naringenin glycosides, apigenin-7-glucoside, quercetin- and kaempferol-3-glucoside were identified. In HE naringenin, apigenin and kaempferol were identified. The amount of naringenin in HE varied from 248.6–757.3mg/g DW while the amount of apigenin varied from 19.6–88.7mg/g DW. Kaempferol was detected in five of thirteen HE and(between 4.5–96.5mg/g DW). Total phenol content (gallic acid equivalent) was estimated using the Folin-Ciocalteu's reagent in NHE. The amount of total phenolics varied from 101.1–266.9mg GAE/g DW. The highest amounts of flavonoid aglycons were detected in extract obtained by extraction with 40% ethanol (v/v) and re-extraction with ethylacetate/ethanol in ratio 9:1.

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2. Aslan, M. et al. (2007)J. Ethnopharmacol. 109:54–59.

3. Smirnov V. V. et al. (1982) Mikrobiolog. Zhurnal 44:71–72.