Planta Med 2008; 74 - PB125
DOI: 10.1055/s-0028-1084470

Sinulodurin A and B, anticancer diterpenes from the soft coral Sinularia dura

MM Radwan 1, S Manly 1, K El Sayed 3, VB Wali 3, P Sylvester 3, B Awate 3, G Shah 3, S Ross 1, 2
  • 1National Center for Natural Products Research, School of Pharmacy, University of Mississippi, University, MS 38677–1848, USA
  • 2Department of Pharmacognosy, School of Pharmacy, University of Mississippi, University, MS 38677–1848, USA
  • 3Department of Basic Pharmaceutical Sciences, College of Pharmacy, University of Louisiana at Monroe, 700 University Avenue, Monroe, Louisiana, USA

Two new diterpenes, sinulodurin A (1) and sinulodurin B (2) along with two known sterols, 24S-methyl cholesterol and 24-methylene cholesterol were isolated from the soft coral, Sinularia dura. The structures of the new metabolites were determined on the basis of spectroscopic methods and by comparison of NMR data with those of related metabolites [1]. Compounds 1 and 2 showed antiproliferative activity against the highly malignant +SA mammary epithelial cells [2] with an IC50 range of 20–30µM. Sinulodurin A (1) and sinulodurin B (2) also displayed anti-invasive activity against the human highly metastatic prostate cancer PC-3M-CT+ cells in the spheroids disaggregation assay [3]. The antimicrobial activities of the isolates were also tested.

Acknowledgements: Materials provided from the Open and Active Repository of the Natural Products Branch at the National Cancer Institute under the code No. C016103. We are grateful to Dr. Bharathi Avula, for assistance with the HR ESIMS, and Dr. Melissa Jacob, Marsha Wright for conducting the antimicrobial testing. The authors also acknowledge the United States Department of Agriculture, Agriculture Research Services Specific Cooperation Agreement No. 58–6408–2-0009 and the NIH, NIAID, Division of AIDS, Grant NO. AI 27094 for partial support of this work.

References: 1. Yamada, K. et al. (1997). Tetrahedron, 53:4569–4578.

2. McIntyre, B. et al. (2000) PSEBM 224: 292–301.

3. Byers, H. et al. (1991) Am. J. Pathol. 139:423–435.