Planta Med 2008; 74 - PB95
DOI: 10.1055/s-0028-1084440

Inhibitors of bacterial multidrug efflux pumps from the resin glycosides of Ipomoea species

R Pereda-Miranda 1, L Chérigo 1, M Fragoso-Serrano 1, N Jacobo-Herrera 1, GW Kaatz 2, S Gibbons 3
  • 1Facultad de Química, Universidad Nacional Autónoma de México, Ciudad Universitaria, CP 04510 DF, México
  • 2Department of Internal Medicine, Division of Infectious Diseases, John D. Dingell Veterans Affairs Medical Centre and Wayne State University School of Medicine, Detroit, Michigan 48201, U.S.A
  • 3Centre for Pharmacognosy and Phytotherapy, The School of Pharmacy, University of London, 29–39 Brunswick Square, London WC1N 1AX, U.K

The CHCl3-soluble extract from Ipomoea murucoides Roemer & Schultes, through preparative-scale recycling HPLC, yielded six new pentasaccharides, murucoidins VI-XI, as well as the known pescaprein III, stoloniferin I and murucoidins I-V. Their structures were characterized through the interpretation of their NMR spectroscopic and FABMS data. All members of the murucoidin series exerted a potentiation effect of norfloxacin against the NorA over-expressing Staphylococcus aureus strain SA-1199B by increasing the activity 4-fold (8µg/mL from 32µg/mL) at concentrations of 5–25µg/mL. Therefore, this type of compounds could be developed to provide potent inhibitors of this multidrug efflux pump.

Acknowledgement: Consejo Nacional de Ciencia y Tecnología (45861-Q) and DGAPA, UNAM (IN208307).