Protective effect of Apium graveolens against experimental myocardial oxidative-stress induced injury in rats
The protective effect of methanolic extract of fruits of Apium graveolens (AGM) was evaluated using adriamycin-induced cardiotoxicity in experimental rats. AGM was administered orally to Sprague-Dawley rats in two different doses (250mg/kg, 500mg/kg) for 21 days. The myocardial oxidative stress induced by single dose of adriamycin (10mg/kg i.p.) decreased levels of marker enzymes such as alanine transaminase (ALT), aspartate transaminase (AST), lactate dehydrogenase (LDH) and creatine kinase (CK) in heart homogenate with concomitant increase of these enzyme levels in serum. The activities of myocardial antioxidant enzymes like superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione reductase (GR) and glutathione-S-transferase (GST) were also significantly reduced by adriamycin treatment. The myocardial reduced glutathione (GSH) was significantly decreased with consequent increase in lipid peroxidation (TBARS) due to adriamycin administration. Pretreatment with AGM 250mg/kg and 500mg/kg p.o. significantly inhibited the alterations in the aforementioned biochemical parameters. One-way ANOVA followed by Bonferroni test was applied to test statistical significance. The histopathology revealed that AGM pretreatment inhibited adriamycin-induced degenerative changes and cellular infiltrations in heart in dose dependent manner. The results demonstrated that pretreatment with Apium graveolens may be useful in preventing the cardiac damage induced by adriamycin in rat heart by virtue of its antioxidant potential.