Effect of wheatgrass (Triticum aestivum L.) on oxidant/antioxidant status in Baf 3p210 and T315i cell lines

In this study, it was aimed to investigate possible effects of aqueous extract of wheatgrass (Triticum aestivum L.) on oxidant/antioxidant status in Baf3p210 (mouse myeloid cell, glivec sensitive type) cell line and T315I cell line (glivec resistant type). For this aim, aqueous extract (200% w/v) was added into the cell line media at final concentration 10%. Beginning, 24^th and 48^th hours' oxidant (malondialdehyde-MDA level and xanthine oxidase-XO activity) and antioxidant (superoxide dismutase-SOD and catalase-CAT activities) parameters and ADA (Adenosine deaminase) activity were measured in the cell line. It was observed that these extracts caused inhibition of ADA activity at beginning but increased MDA levels, SOD and CAT activities in the cell line. In conclusion, it has been suggested that the extract directly causes oxidant stress in cell lines owing to its own oxidant ingredients. It seemed that this compensatory change could not prevent the oxidant stress created. We think that the oxidant potential of the extract might play part in its possible anticancer potential supposed by several investigators previously.