Planta Med 2008; 74 - PA259
DOI: 10.1055/s-0028-1084257

Development of novel in situ Turmeric gel for the treatment of Inflammatory Bowel Disease

SS Gilda 1, SS Baheti 1, AR Paradkar 1
  • 1Bharati Vidyapeeth University, Poona College Of Pharmacy, Erandwane, Pune-411038, Maharashtra, India

Inflammatory bowel disease (IBD) is characterized by oxidative and nitrosative stress, leukocyte infiltration and up regulation of immune system. The aim of the present study was to investigate protective effects of in situ turmeric gel on TNBS induced colitis, a model for IBD. Poloxamer 407, a non-ionic copolymer exhibiting temperature dependent gelation was used as a gelling agent. Final formulation was characterized on the basis of plane polarized microscopy, rheology and release study. Further dose – response studies revealed that pretreatment of rat with formulation (50mg of curcumin/kg; daily for 10 days) significantly ameliorated the appearance of diarrhoea and the disruption of colonic architecture. It was also noted that there was significant reduction in degree of neutrophil infiltration (measured as myleoperoxidase activity) and lipid peroxidation (measured as decrease in malondialdehyde activity) in formulation treated group. Also reduced levels of nitric oxide were found. These findings suggests further step towards formulation development for IBD.

Table 1. Effect of curcumin and formulation administration on colonic cytoprotection following administration of TNBS.

Colonic erosion

Groups

Scale

n

mean

0

1

2

3

4

Control

4

2

0

0

0

6

0.40

TNBS

0

1

1

2

2

6

2.60

Curcumin (50mg/kg)

0

4

1

1

0

6

1.50

Formulation

0

4

2

0

0

6

1.40

Acknowledgements:The Vice-chancellor, Bharati Vidyapeeth University and Principal Poona College of Pharmacy, Pune-411038. India.