Antiproliferative and cytotoxic activity of infusions from four Staphylea L. species

Ľ Laciková; M. Mrižová; I. Mašterová; D. Grančai; M. Ficková

doi:10.1055/s-0028-1084216

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Planta Med 2008; 74 - PA218
DOI: 10.1055/s-0028-1084216

Antiproliferative and cytotoxic activity of infusions from four Staphylea L. species

Ľ Laciková ¹, M Mrižová ¹, I Mašterová ¹, D Grančai ¹, M Ficková ²

¹Department of Pharmacognosy and Botany, Faculty of Pharmacy, Comenius University, Odbojárov 10, 832 32 Bratislava, Slovakia
²Institute of Experimental Endocrinology SAV, Vlárska 3, 83306 Bratislava Slovakia

Congress Abstract

Water infusions from some Staphylea L. species (Staphyleaceae) are used in Traditional Chinese Medicine. Based on this fact, time (24 and 72h) and dose (1–150µg/ml) dependent antiproliferative and toxic effects of water leaf infusions from S. colchica (SC), S. elegans (SE), S. holocarpa (SH) and S. pinnata (SP) were investigated. Human skin carcinoma cells A431 were used for determination of antiproliferative activity/cell viability (MTT test) and cytotoxic effects were measured by LDH leakage (representing membrane integrity). Screening of these activities has shown time dependent increase of destructive effects, with SE performing the best. The ED₅₀values for the mitochondrial and membrane integrity damage were quite similar for SE extract. This finding indicates simultaneous harmful effect on both cell compartments. SC, SH and SP infusions display similar ED₅₀ values for intracellular and membrane damage as well as the extent of cell growth inhibition and LDH leakage. The values represent mean±SE of 4 separate experiments performed in duplicate. Statistical significance was calculated using Student's t-test and the one-way ANOVA test, when *p<0.05; **p<0.01; ***p<0.0001 (24h vs. 72h).

Species	Assay	Parameter	24h	72h	Species	Assay	Parameter	24h	72h
SC	MTT	ED₅₀ (µg/ml)±SE	20.1±1.3	5.9±0.9***	SH	MTT	ED₅₀ (µg/ml)±SE	38.9±3.0	16.3±1.1***
		max. inhibition (%)	60.9±11.1	37.2±1.2			max. inhibition (%)	66.6±2.3	40.2±0.6***
	LDH	ED₅₀ (µg/ml)±SE	32.4±1.6	23.3±2.4**		LDH	ED₅₀ (µg/ml)±SE	38.3±2.3	29.5±2.0*
		max. inhibition (%)	32.1±4.9	12.9±2.3*			max. inhibition (%)	32.5±4.1	14.4±0.9**
SE	MTT	ED₅₀ (µg/ml)±SE	18.3±1.5	10.8±1.9*	SP	MTT	ED₅₀ (µg/ml)±SE	35.3±3.8	12.6±1.9***
		max. inhibition (%)	43.6±2.8	33.7±1.2*			max. inhibition (%)	50.4±3.8	30.9±2.5*
	LDH	ED₅₀ (µg/ml)±SE	19.6±1.3	13.7±2.1*		LDH	ED₅₀ (µg/ml)±SE	23.0±1.3	17.5±1.6*
		max. inhibition (%)	24.4±1.7	10.5±3.4*			max. inhibition (%)	20.1±1.8	21.2±0.7

Acknowledgements: Institute of Experimental Endocrinology SAV, Grant VEGA SR 1/4289/07