Planta Med 2008; 74 - PA212
DOI: 10.1055/s-0028-1084210

Effect of Curculigo orchioides Gaertn on Compound 48/80 induced systemic anaphylaxis in mice

P Venkatesh 1, PK Mukherjee 1, A Bandyopadhyay 2
  • 1School of Natural Product Studies, Department of Pharmaceutical Technology, Jadavpur University, Kolkata-700032, India
  • 2Department of Molecular Endocrinology, Indian Institute of Chemical Biology, Jadavpur, Kolkata-700032, India

The aim of this work was to study the effect of plant Curculigo orchioides Gaertn (Rhizomes, COR) on Compound 48/80 induced systemic anaphylaxis and inhibition of histamine release in the blood. The original stock of ICR mice were acclimatized for a week in standard conditions. Toxicity profile: COR (methanolic) is found to be up to 10g/kg as non-toxic. Compound 48/80 induced systemic anaphylaxis: The mortality rate was observed on the Compound 48/80 (mast cell degranulator) treated group (n=10/group) and on drug treated groups. COR was administered orally from 0.1 to 0.4g/ml, 1h before the injection of Compound 48/80. Mortality was monitored for 1h after induction of anaphylactic shock (Table 1) and then blood sample were analyzed from each mouse heart [1]. Preparation of plasma and histamine determination: The Blood was centrifuged at 400Xg for 10min. Plasma was withdrawn and histamine content was measured by o-phthlaldehyde spectrofluorimetric procedure [2]. The fluorescent intensity was measured at 438nm (excitation at 353nm). The lower rate of mortality of mice from the anaphylactic shock was 30% at 0.4g/kg/b.w (COR). The elevation of histamine in the plasma from different groups of mice was determined. The results demonstrated that COR inhibits the mast cell dependent anaphylactic reaction in mice at IC50=0.385g/kg and it is suggestive that the activity may be due to the inhibition of histamine release from the mast cells activation by both IgE dependent and IgE independent stimuli.

Table 1. Effect of COR on Compound 48/80-induced systemic anaphylactic reaction.
* P<0.001; significantly different from the saline value

Treatment

Dose (g/kg)

Compound 48/80 (0.8g/kg)

Mortality (%)

None (Saline)

-

-

0

Toxicant

-

+

100

Ketotifen (Std)

0.1

+

40*

Ketotifen (Std)

0.2

+

0*

COR

0.1

+

90*

COR

0.2

+

60*

COR

0.3

+

50*

COR

0.4

+

30*

Acknowledgements: Financial assistance from Indian Council of Medical Research, Government of India, New Delhi.

References: 1. Yi, J.M. et al. (2001). Int. J. Orient. Med. 2:47. 2. Shore, P.A. et al. (1959)J. Pharmacol. Exp. Ther. 127:182.