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Chemical constituents and antimicrobial activity of Verbena tenuisecta
The volatile constituents of the flowering buds of Verbena tenuisecta Briq. were prepared by hydrodistillation and analyzed by GC/MS. It was found that they consist of a mixture of thirteen compounds in which the main compounds are 1-octen-3-ol (52.87%), bicyclo[3.2.0]-heptan-2-one-6-hydroxy-5-methyl-6-vinyl (13.89%) and limonene (9.33%). Investigation of the flavonoidal constituents resulted in the isolation of luteolin and apigenin from the chloroform fraction, while chrysoeriol-7-O-glucoside, chrysoeriol-7-O-rhamno-arabinoside and isorhamnetin-3-O-rhamno-glucoside were isolated from the butanol fraction of the methanol extract. All compounds were identified by chromatographic and spectroscopic methods (UV, MS, 1H and 13C NMR spectroscopy). The antimicrobial activity of different extracts against some selected organisms was evaluated by measuring the inhibition zone diameter (IZ). The results revealed that methanol extracts exhibited the highest inhibition against Mycobacterium phlei while butanol extracts showed moderate activity against Candida albicans and Staphylococcus aureus .
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