The effect of White Kwao Krua [Pueraria candollei Grah. var. mirifica (Airy Shaw et Suvatabandhu) Niyomdham] crude extract containing puerarin on vascular relaxation in the White Rat (Rattus norvegicus)
The tuberous roots of the White Kwao Krua [Pueraria candollei Grah. var. mirifica (Airy Shaw et Suvatabandhu) Niyomdham] significantly accumulate isoflavone glucosides such as puerarin. The flavonoids can promote vascular relaxation . The purpose of this study was to increase puerarin accumulation in the tuberous roots of White Kwao Krua (WKK) and the effect of WKK crude extract on vascular relaxation in the White Rat (Rattus norvegicus) was investigated. The experiment was a RCBD with 4 replications and 5 treatments of Zn2+ concentration levels. The WKK were sprayed with Zn2+ at the concentration of 0 (distilled water), 50, 100, 200 and 300mg/L. TLC and HPLC techniques [2,3,4] were used to identify the amount of puerarin in the tuberous roots of WKK. The vascular relaxation in the White Rat was investigated according to the method of Longbottom et al. (2000) . The concentrations of Zn2+ studied had a statistically significant effect on the amount of puerarin. Zn2+ at 200mg/L gave the highest amount of puerarin (194.3µg/g dry weight). The blood vessels of the white rats that were treated with WKK crude extract at every treatment resulted in highly significant effects on vascular relaxation compared with untreated blood vessels. The blood vessels that were treated with acetylcholine plus WKK crude extract at the concentration 200mg/L gave the highest relaxation. WKK crude extract showed vascular relaxation in White Rats, and spraying Zn2+ onto WKK can increase puerarin in tuberous roots.
Acknowledgements: Suranaree University of Technology and the National Research Council of Thailand (NRCT)
References: 1. Benlhabib, E. et al. (2004)J of Medicinal Food. 7: 180–186. 2. Li, M. et al. (2003) Fenxi Huaxue. 31:178–180. 3. Li, D. et al, (2004) Carbohydrate Research. 339: 2789–2797. 4. Chalardkid, P. et al (2003) Suranaree J Sci. Technol. 10:350–358. 5. Longbottom, E. et al. (2000) Europe J Physiology 440:315–321.