Reversible contraceptive effect of isolated fractions of Cuminum cyminum (Linn.) seed extract on reproductive functions of male rats

In the present study proven fertile male rats were treated with 50:50 isolated fraction (Chloroform: Methanol) of Cuminum cyminum seed at 50mg/rat/day for 60 days. On day 61 animals were autopsied, testes and accessory sex organs i.e. epididymides, seminal vesicle, ventral prostate were dissected out and weighed. The sperm motility and density were significantly reduced in the treated group. Testicular cell dynamical studies showed that the number of spermatogenic elements i.e. spermatogonia, preleptotene, pachytene, secondary spermatocyte and round spermatids were decline in fraction treated group. The number of mature Leydig cells was decreased, whereas the degenerating Leydig cells were increased. The total protein, sialic acid contents of the testes, epididymides, seminal vesicle and prostate were reduced. Testicular glycogen content was decreased whereas cholesterol content of testes elevated. Testosterone levels were declined significantly. The values of RBC, WBC, haemoglobin, haematocrit were found to be within the normal range. In conclusion the 50:50 isolated fraction (CHCl₃:CH₃OH) of C. cyminum methanol seed extract in the dose level of 50mg/rat/day effects reproductive function in male rats.