Planta Med 2008; 74 - PA96
DOI: 10.1055/s-0028-1084094

Effects of organic and inorganic compounds isolated of Argemone mexicana on the perfusion pressure of the mesenteric vascular bed

JL Escobar 1, J Cassani 2, R Reyes-Chilpa 3, GA Magos 1
  • 1Facultad de Medicina. Departamento de Farmacología, UNAM, Coyoacán 04510, México, D.F
  • 2Universidad Autónoma Metropolitana. Unidad Xochimilco Calz. del Hueso 1100, Col. Villa Quietud, Coyoacán 04960México, D.F
  • 3Instituto de Química, UNAM, Coyoacán 04510, México, D. F

In a previous study, we found that the methanolic extract (ME) from the aerial parts of Argemone mexicana has vasoconstrictor and vasodilator effects in isolated aortic rings of rat [1]. In the present work, these effects were characterized on a perfused mesenteric artery preparation (MAP) of rat and were correlated with the effects on the cardiovascular system of the anesthetized rats. ME from A. mexicana significantly increased the perfusion pressure of MAP. On the other hand, the cumulative concentration-response curve from norepinephrine diminished without affecting the slope in the KCl curve. Two active compounds with antagonic effects were isolated from the ME through biodirected fractionation. The substance that increases the perfusion pressure in the MAP was identified as KNO3 by IR, atomic-absorption spectroscopy, as well as by analytical reactions to determine K+ and NO3 - ions. This effect can be explained since KNO3 induces a calcium flow toward the intracellular compartment. The substance that diminished the perfusion pressure of the MAP was positive with Dragendorff and Mayer reactions and belongs to the protoberberine alkaloid group, according to its 1H and 13C NMR, MS, and IR data. The action mechanism of this alkaloid does not seem to influence the increment in the calcium flow.

Acknowledgements: UNAM, PAPIIT. CLAVE: IN211508–2

Reference: 1. Páez-Sánchez, E. et al. (2006). Proc. West. Pharmacol. Soc. 49: 63–65