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Anti-inflammatory and antioxidant activities of Haplopappus taeda extracts
Haplopappus taeda (“Bailahuén“), is a native medicinal Haplopappus (Asteraceae) species included within a“Bailahuén complex“, frequently used in folk medicine for liver ailments. The aim of this work was to show that this pharmacological effect could be associated with anti-inflammatory properties of secondary metabolites present in the leaves. To this purpose, the ethanolic extract of fresh plant (Ht) was submitted to assay-guided chromatographic fractionation to give three main active compounds that were identified by spectroscopic methods. Anti-inflammatory effect was evaluated in vivo using the AA-induced acute ear oedema  assay (20µL, 3.6×10-4M/ear) along with in vitro assays (free radical scavenging with DPPH , Xanthine oxidase inhibition and superoxide anion scavenging ). The results show a high anti-inflammatory activity of Ht (72.7%±23.1) that derives mainly from the monoterpene taedol (45.7%±11.2), the clerodane diterpene ent-5α-18-acetoxy-cis-cleroda-3,13 E-dien-15-oic acid (30.1%±12) and the flavanone sakuranetin (34.3%±11.5). Under the same experimental conditions, nimesulide displayed a maximum of 48.8%±4 topical anti-inflamatory effect. Significance of results was calculated using Wilcoxon's non parametric method for independent data. Effects calculated are relative to the mean value±S.E.M.
Aknowledgements : FIA (ES-C-2005–1-A-007), Facultad de Ciencias Universidad de Chile and Fondecyt (P 1030813).
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