Planta Med 2008; 74 - PA72
DOI: 10.1055/s-0028-1084070

The in vitro screening for hypoglycemic and antihypertensive activities of Dipsacus fullonum L

F Tillequin 1, R Tundis 2, B Deguin 1, MR Loizzo 2, M Bonesi 2, F Menichini 2, F Conforti 2, M Marrelli 2, G Statti 2, F Menichini 2
  • 1Laboratoire de Pharmacognosie de l'Université René Descartes, U.M.R./C.N.R.S. No. 8638, Faculté des Sciences Pharmaceutiques et Biologiques, 4, Avenue de l'Observatoire, F-75006 Paris, France
  • 2Department of Pharmaceutical Sciences, Faculty of Pharmacy and Nutrition and Health Sciences, University of Calabria, I-87036 Arcavata di Rende (CS), Italy

The aim of the present study was to evaluate the hypoglycaemic and antihypertensive activities of Dipsacus fullonum L. (Dipsacaceae). The genus Dipsacus characteristically contains iridoid glucosides [1], triterpene glycosides [2], alkaloids [3] and other constituents [4]. The aerial parts of D. fullonum (1.056kg) were exhaustively extracted with methanol at room temperature (yield 7.59%). In order to separate the chemical compounds in function of their polarity, the crude extract was dissolved in distilled water and partitioned with n-hexane (yield 0.41%), chloroform (yield 0.5%), ethyl acetate (yield 0.32%) and n-butanol (yield 1.27%). The Angiotensin Converting Enzyme (ACE) inhibitory activity was measured trough the cleavage of the chromophore-fluorophore labelled substrate dansyltriglycine by ACE preparation from rabbit lung (EC into dansylglycine which is quantitatively measured by HPLC. The most effective fractions were obtained in chloroform and n-butanol which gave 74.68% and 75.35% inhibition at 300µg/ml. Hypoglycaemic effects were examined using the in vitro assay based on the inhibition of α-amylase. Inhibitors of this enzymes delay carbohydrate digestion and prolong overall carbohydrate digestion time, causing a reduction in the rate of glucose absorption and consequently blunting the post-prandial plasma glucose rise. The ethyl acetate fraction was the most active with an IC50 value 48.44µg/ml. The inhibitory activity of n-hexane and chloroform fractions was weaker with IC50 values of 310.66 and 293.82µg/ml, respectively. Further fractionations will be required to identify the major active constituents.

References: 1. Kocsis, A. et al. (1993)J. Nat. Prod. 56:1486.

2. Tomita, H. et al. (1996) Phytochemistry 42:239.

3. Rakhmatullaev, TU. et al. (1972) Khimiya Prirodnykh Soedinenii 3:400.

4. Abdallah, OM. (1991) Phytochemistry 30:2805.