Planta Med 2008; 74 - PA63
DOI: 10.1055/s-0028-1084061

Antioxidant and anti-inflammatory activity of Cotinus coggygria extracts

M Simić 1, D Vučićević 2, M Milenković 2, N Kovačević 1
  • 1Institute of Pharmacognosy, Faculty of Pharmacy, V. Stepe 450, 11221 Belgrade, Serbia
  • 2Institute of Microbiology and Immunology, Faculty of Pharmacy, V. Stepe 450, 11221 Belgrade, Serbia

Aim of this study was the investigation of antioxidant and anti-inflammatory activities of the smoke tree, Cotinus coggygria Scop., Anacardiaceae. Ground, dried young shoots were extracted with acetone and then fractionated with chloroform, ethyl-acetate and water (yield of extracts: 42.5%, 7.3%, 53.0% and 39.7%, respectively). Content of total phenolics and tannins was determined using Folin-Ciocalteu reagent and total flavonoids on basis of flavonoid-aluminium complex formation [1]. Antioxidant activity was evaluated with FRAP test [2], DPPH radical scavenging activity [3] and lipid peroxidation (LP) test [4]. The carrageenan-induced rat paw oedema test was used for investigation of anti-inflammatory activity [5]. Highest content of total phenolics (92.9%), tannins (83.4%) and flavonoids (3.5%) was determined in ethyl-acetate fraction. In this fraction gallic acid, apigenine, luteoline and their derivatives were detected by HPLC. All extracts showed antioxidant effects but the highest activity was obtained with the ethyl-acetate fraction. This fraction also exhibited significant ferric reducing ability (5.0 mmol Fe 2+/g extract), very high DPPH radical scavenging activity (SC50=1.7µg/ml) and high inhibition of LP on liposomes (IC50=41.8µg/ml). Furthermore, it reduced the carrageenan rat paw oedema in a dose-dependent manner; doses of 50mg/kg and 100mg/kg led to a 46.5% and 76.7% reduction of the oedema, being statistically very significant (p<0.01). At a dose of 100mg/kg the anti-inflammatory effect of the ethyl-acetate fraction was more pronounced than that of indomethacin (8mg/kg; p<0.01).

References: 1. European Pharmacopoeia, 6th Edition (2008). Council of Europe. Strasbourg. 2. Szőllősi, R. et al. (2002) Act Biol Szeg 46:125–7. 3. Bondet, V. et al. (1997) Lebensm.-Wiss. u.-Technol. 30:609–15. 4. Afanas'ev, I. B. et al. (1989) Biochem. Pharmacol. 38: 1763–9. 5. Oyanagui, Y., et al. (1991) Arzneimittelforschung Drug Res. 41:5.